Application

Immunohistochemistry at 2 µg/mL on tissues fixed with cold acetone

Immunocytochemistry at 2 µg/mL on U251 cells

Immunoblotting (Western) at 2 µg/mL to detect whole tenascin under reducing conditions

ELISA: 2 x 10-10 M endpoint dilution

Affinity constant Kd= 6.37 x 10-9

Final working dilutions must be determined by end user.

This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.

Immunogen

Epitope: type III repeats 1-3

Purified human tenascin

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.

Format: Purified

Specificity

Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.

Storage and Stability

Store at 2 and 8°C for up to 6 months.